DivIVA

• Description: cell-division initiation protein (septum placement)
  
  

Gene name	divIVA
Synonyms	ylmJ
Essential	no
Product	cell-division initiation protein
Function	septum placement
MW, pI	19 kDa, 4.846
Gene length, protein length	492 bp, 164 aa
Immediate neighbours	ylmH, ileS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15420

Phenotypes of a mutant

Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.

• Protein family: gpsB family (according to Swiss-Prot)

• Paralogous protein(s): GpsB

Extended information on the protein

• Kinetic information:

• Domains: The first 60 amino acids constitute a lipid binding domain. PubMed

Multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain.

• Modification: The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DivIVA-RacA, DivIVA-MinJ, DivIVA-DivIVA

• Localization: DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles.

Database entries

• Structure:

• UniProt: P71021

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: one gene cistron

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: divIVA::tet available from the Hamoen Lab

• Expression vector:

• lacZ fusion:

• GFP fusion:divIVA-gfp fusions available from the Hamoen Lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [4]

Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage