Difference between revisions of "RibH"

From SubtiWiki
Jump to: navigation, search
Line 90: Line 90:
 
* '''Operon:'''  ''[[ypuE]]-[[ribD]]-[[ribE]]-[[ribA]]-[[ribH]]-[[ribT]]''  [http://www.ncbi.nlm.nih.gov/sites/entrez/8159171 PubMed]
 
* '''Operon:'''  ''[[ypuE]]-[[ribD]]-[[ribE]]-[[ribA]]-[[ribH]]-[[ribT]]''  [http://www.ncbi.nlm.nih.gov/sites/entrez/8159171 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8159171 PubMed]
+
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190}}, [[SigA]] {{PubMed|8159171}}
  
 
* '''Regulation:''' expressed in the absence of FMN ([[FMN-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15808508 PubMed]
 
* '''Regulation:''' expressed in the absence of FMN ([[FMN-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15808508 PubMed]

Revision as of 15:37, 31 July 2009

  • Description: riboflavin synthase (beta subunit)

Gene name ribH
Synonyms
Essential no
Product riboflavin synthase (beta subunit)
Function riboflavin biosynthesis
MW, pI 16 kDa, 5.196
Gene length, protein length 462 bp, 154 aa
Immediate neighbours ribT, ribA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RibH context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU23250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)
  • Protein family: DMRL synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References