Difference between revisions of "Rnz"
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2FK6 2FK6] (in complex with tRNA-Thr), [http://www.rcsb.org/pdb/explore.do?structureId=1Y44 1Y44] | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2FK6 2FK6] (in complex with tRNA-Thr), [http://www.rcsb.org/pdb/explore.do?structureId=1Y44 1Y44] | ||
| − | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/P54548 P54548] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23840] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23840] | ||
Revision as of 13:28, 20 July 2009
- Description: RNase Z
| Gene name | rnz |
| Synonyms | yqjK |
| Essential | yes PubMed |
| Product | endoribonuclease Z |
| Function | processing of CCA-less tRNA precursors |
| MW, pI | 33 kDa, 5.805 |
| Gene length, protein length | 921 bp, 307 aa |
| Immediate neighbours | yqjL, zwf |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU23840
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs (according to Swiss-Prot) processing of CCA-less tRNA precursors
- Protein family: RNase Z family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: P54548
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971]
[WorldCat.org]
[DOI]
(P p)
Inés Li de la Sierra-Gallay, Nathalie Mathy, Olivier Pellegrini, Ciarán Condon
Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA.
Nat Struct Mol Biol: 2006, 13(4);376-7
[PubMed:16518398]
[WorldCat.org]
[DOI]
(P p)
Inés Li de la Sierra-Gallay, Olivier Pellegrini, Ciarán Condon
Structural basis for substrate binding, cleavage and allostery in the tRNA maturase RNase Z.
Nature: 2005, 433(7026);657-61
[PubMed:15654328]
[WorldCat.org]
[DOI]
(I p)
Olivier Pellegrini, Jamel Nezzar, Anita Marchfelder, Harald Putzer, Ciarán Condon
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis.
EMBO J: 2003, 22(17);4534-43
[PubMed:12941704]
[WorldCat.org]
[DOI]
(P p)
