MurB

• Description: UDP-N-acetylenolpyruvoylglucosamine reductase
  
  

Gene name	murB
Synonyms	ylxC
Essential	yes PubMed
Product	UDP-N-acetylenolpyruvoylglucosamine reductase
Function	peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Cell wall
MW, pI	32 kDa, 6.087
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	murG, divIB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15230

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetylmuramate + NADP⁺ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH (according to Swiss-Prot)

• Protein family: FAD-binding PCMH-type domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P18579

• KEGG entry: [3]

• E.C. number: 1.1.1.158

Expression and regulation

• Operon: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp

• Sigma factor: SigE
• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage

References

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)

Gonçalo Real, Adriano O Henriques
Localization of the Bacillus subtilis murB gene within the dcw cluster is important for growth and sporulation.
J Bacteriol: 2006, 188(5);1721-32
[PubMed:16484183] [WorldCat.org] [DOI] (P p)

S L Rowland, J Errington, R G Wake
The Bacillus subtilis cell-division 135-137 degrees region contains an essential orf with significant similarity to murB and a dispensable sbp gene.
Gene: 1995, 164(1);113-6
[PubMed:7590298] [WorldCat.org] [DOI] (P p)