Difference between revisions of "LigA"
| Line 80: | Line 80: | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1B04 1B04] (adenylation domain, Geobacillus stearothermophilus) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1B04 1B04] (adenylation domain, Geobacillus stearothermophilus) | ||
| − | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/O31498 O31498] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU06620] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU06620] | ||
Revision as of 10:52, 20 July 2009
- Description: DNA ligase (NAD-dependent)
| Gene name | ligA |
| Synonyms | lig, yerG |
| Essential | yes PubMed |
| Product | DNA ligase (NAD-dependent) |
| Function | replication |
| MW, pI | 74 kDa, 4.708 |
| Gene length, protein length | 2004 bp, 668 aa |
| Immediate neighbours | pcrA, yerH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU06620
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)
- Protein family: BRCT domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)
- UniProt: O31498
- KEGG entry: [3]
- E.C. number: 6.5.1.2
Additional information
Expression and regulation
- Operon:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290]
[WorldCat.org]
[DOI]
(P p)
M A Petit, S D Ehrlich
The NAD-dependent ligase encoded by yerG is an essential gene of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(23);4642-8
[PubMed:11095673]
[WorldCat.org]
[DOI]
(I p)
