YabJ

• Description: required for activity of PurR
  
  

Gene name	yabJ
Synonyms
Essential	no
Product	unknown
Function	regulation of purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	13 kDa, 5.134
Gene length, protein length	375 bp, 125 aa
Immediate neighbours	purR, spoVG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00480

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Ref.3 (according to Swiss-Prot) YjgF family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: forms a trimer PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1QD9, 1QD9 NCBI PubMed

• UniProt: P37552

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: purR-yabJ PubMed

• Sigma factor:

• Regulation: negative autoregulation PubMed
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism: repression by PurR PubMed
  • PurR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S Sinha, P Rappu, S C Lange, P Mäntsälä, H Zalkin, J L Smith
Crystal structure of Bacillus subtilis YabJ, a purine regulatory protein and member of the highly conserved YjgF family.
Proc Natl Acad Sci U S A: 1999, 96(23);13074-9
[PubMed:10557275] [WorldCat.org] [DOI] (P p)

P Rappu, B S Shin, H Zalkin, P Mäntsälä
A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor.
J Bacteriol: 1999, 181(12);3810-5
[PubMed:10368157] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)