Difference between revisions of "IspF"
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[yacL]]-[[ispD]]-[[ispF]]'' {{PubMed|10482513}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] |
| − | * '''Regulation:''' | + | * '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
| Line 119: | Line 119: | ||
=References= | =References= | ||
| + | |||
| + | <pubmed>15805528, 10482513 </pubmed> | ||
Revision as of 20:56, 13 July 2009
- Description: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
| Gene name | ispF |
| Synonyms | yacN |
| Essential | yes PubMed |
| Product | 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase |
| Function | isoprenoid biosynthesis |
| MW, pI | 16 kDa, 5.398 |
| Gene length, protein length | 474 bp, 158 aa |
| Immediate neighbours | ispD, gltX |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU00910
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)
- Protein family: ispF family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: Q06756
- KEGG entry: [3]
- E.C. number: 4.6.1.12
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528]
[WorldCat.org]
[DOI]
(P p)
A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513]
[WorldCat.org]
[DOI]
(P p)
