Difference between revisions of "Sandbox"

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−
* '''Description:''' aminoglycoside 6-adenylyltransferase <br/><br/>
+
* '''Description:''' flagella-associated protein, signal recognition particle-GTPase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''aadK''
+
|''flhF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || aminoglycoside 6-adenylyltransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || signal recognition particle-GTPase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to streptomycin
+
|style="background:#ABCDEF;" align="center"|'''Function''' || placement and assembly of polar flagella
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.755  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 8.719  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 852 bp, 284 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1098 bp, 366 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrdA]]'', ''[[yrpB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhA]]'', ''[[swrC]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14620&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13513&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:aadK_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:flhF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU26790
+
* '''Locus tag:''' BSU16400
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11037]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10544]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:'''
+
* '''Protein family:''' GTP-binding SRP family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:'''
+
* '''Localization:''' cell membrane (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PBE 2PBE]
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PX3 2PX3] (complexed with GTP-Mg2+)
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P17585 P17585]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q01960 Q01960]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26790]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16400]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
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=References=
 
=References=
  
−
<pubmed>2550327,8293959,, </pubmed>
+
<pubmed>1447978,17699634,,15317803,14651647, </pubmed>

Revision as of 15:48, 2 July 2009

  • Description: flagella-associated protein, signal recognition particle-GTPase

Gene name flhF
Synonyms
Essential no
Product signal recognition particle-GTPase
Function placement and assembly of polar flagella
MW, pI 40 kDa, 8.719
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours flhA, swrC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FlhF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU16400

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: GTP-binding SRP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 2PX3 (complexed with GTP-Mg2+)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Gert Bange, Georg Petzold, Klemens Wild, Richard O Parlitz, Irmgard Sinning
The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP.
Proc Natl Acad Sci U S A: 2007, 104(34);13621-5
[PubMed:17699634] [WorldCat.org] [DOI] (P p)

Geeske Zanen, Haike Antelmann, Helga Westers, Michael Hecker, Jan Maarten van Dijl, Wim J Quax
FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion.
J Bacteriol: 2004, 186(17);5956-60
[PubMed:15317803] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

P B Carpenter, D W Hanlon, G W Ordal
flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP-binding protein.
Mol Microbiol: 1992, 6(18);2705-13
[PubMed:1447978] [WorldCat.org] [DOI] (P p)

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