Difference between revisions of "Sandbox"

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* '''Description:''' Hallo Jörg, das Programm zur Reparatur der KEGG-Links steht. Da jedoch meine KEGG-Genliste schon einige Tage alt ist, gleiche ich sie zuvor mit der jetzt bestehenden SubtiWiki-Hauptseitenversion ab. Danach findet dann die Reparatur statt. <br/><br/>
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* '''Description:''' aminoglycoside 6-adenylyltransferase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''fmt''
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|''aadK''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloL ''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || methionyl-tRNA formyltransferase
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|style="background:#ABCDEF;" align="center"| '''Product''' || aminoglycoside 6-adenylyltransferase
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || formylation of Met-tRNA(fMet)
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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to streptomycin
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging]'''
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.755 
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.618 
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 852 bp, 284 aa
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 951 bp, 317 aa
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrdA]]'', ''[[yrpB]]''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[def]]'', ''[[yloM]]''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14620&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13446&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|colspan="2" | '''Genetic context''' <br/> [[Image:aadK_context.gif]]
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|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:fmt_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
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<br/><br/><br/><br/><br/><br/>
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<br/><br/>
  
 
=The gene=
 
=The gene=
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=== Basic information ===
 
=== Basic information ===
  
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* '''Locus tag:''' BSU15730
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* '''Locus tag:''' BSU26790
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
−
 
−
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
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* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11937]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11037]
  
 
=== Additional information===
 
=== Additional information===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' 10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H<sub>2</sub>O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet) (according to Swiss-Prot)
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* '''Catalyzed reaction/ biological activity:'''  
  
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* '''Protein family:''' fmt family (according to Swiss-Prot)
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* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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=== Database entries ===
 
=== Database entries ===
  
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* '''Structure:'''
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PBE 2PBE]
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94463 P94463]
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P17585 P17585]
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15730]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26790]
  
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* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.2.9 2.1.2.9]
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* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:''' ''[[def]]-[[fmt]]-[[yloM]]'' [http://www.ncbi.nlm.nih.gov/pubmed/16964327?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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* '''Operon:'''  
  
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/16964327?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
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* '''Additional information:'''
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* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
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<pubmed>16964327,,19171795, 19171795 </pubmed>
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<pubmed>2550327,8293959,, </pubmed>

Revision as of 00:53, 25 June 2009

  • Description: aminoglycoside 6-adenylyltransferase

Gene name aadK
Synonyms
Essential no
Product aminoglycoside 6-adenylyltransferase
Function resistance to streptomycin
MW, pI 33 kDa, 4.755
Gene length, protein length 852 bp, 284 aa
Immediate neighbours yrdA, yrpB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AadK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU26790

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

N Noguchi, M Sasatsu, M Kono
Genetic mapping in Bacillus subtilis 168 of the aadK gene which encodes aminoglycoside 6-adenylyltransferase.
FEMS Microbiol Lett: 1993, 114(1);47-52
[PubMed:8293959] [WorldCat.org] [DOI] (P p)

K Ohmiya, T Tanaka, N Noguchi, K O'Hara, M Kono
Nucleotide sequence of the chromosomal gene coding for the aminoglycoside 6-adenylyltransferase from Bacillus subtilis Marburg 168.
Gene: 1989, 78(2);377-8
[PubMed:2550327] [WorldCat.org] [DOI] (P p)

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