Difference between revisions of "PyrC"

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|style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.427   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.427   

Revision as of 14:15, 18 June 2009

  • Description: dihydroorotase

Gene name pyrC
Synonyms
Essential no
Product dihydroorotase
Function pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 46 kDa, 5.427
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours pyrB, pyrAA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15500

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate (according to Swiss-Prot)
  • Protein family: Type 2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s): PucH

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References