Difference between revisions of "AcsA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of acetate, fatty acids  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of acetate, fatty acids  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.547   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.547   

Revision as of 12:13, 11 June 2009

  • Description: acetyl-CoA synthetase

Gene name acsA
Synonyms
Essential no
Product acetyl-CoA synthetase)
Function utilization of acetate, fatty acids
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 64 kDa, 5.547
Gene length, protein length 1716 bp, 572 aa
Immediate neighbours tyrS, acuA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AcsA context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Locus tag: BSU29680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: acsA
  • Regulation: repressed by glucose (4.5-fold) (CcpA) PubMed, repressed by CodY PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jeffrey G Gardner, Jorge C Escalante-Semerena
In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase.
J Bacteriol: 2009, 191(6);1749-55
[PubMed:19136592] [WorldCat.org] [DOI] (I p)

Jeffrey G Gardner, Jorge C Escalante-Semerena
Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl coenzyme a synthetase (AcsA) in Bacillus subtilis.
J Bacteriol: 2008, 190(14);5132-6
[PubMed:18487328] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)


  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed