Difference between revisions of "GlpF"
| Line 13: | Line 13: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || glycerol uptake | |style="background:#ABCDEF;" align="center"|'''Function''' || glycerol uptake | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]''' | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 9.506 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 9.506 | ||
Revision as of 11:45, 11 June 2009
- Description: glycerol facilitator
| Gene name | glpF |
| Synonyms | |
| Essential | no |
| Product | glycerol facilitator |
| Function | glycerol uptake |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 28 kDa, 9.506 |
| Gene length, protein length | 822 bp, 274 aa |
| Immediate neighbours | glpP, glpK |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU09280
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: View classification (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P18156
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: repressed by glucose (8-fold) (CcpA) PubMed, carbon catabolite repression, induction by glycerol
- Regulatory mechanism: CcpA: transcription repression, catabolite repression: repression by CcpA, induction: GlpP-dependent RNA switch (transcriptional antitermination)
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
- Beijer L, Nilsson RP, Holmberg C, Rutberg L: The glpP and glpF genes of the glycerol regulon in Bacillus subtilis. J Gen Microbiol 1993;139:349-359. PubMed
- Darbon E, Servant P, Poncet S, Deutscher J (2002) Antitermination by GlpP, catabolite repression via CcpA and inducer exclusion triggered by P~GlpK dephosphorylation control Bacillus subtilis glpFK expression. Mol Microbiol 43:1039-1052. PubMed
