Difference between revisions of "PyrAB"
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' | + | * '''Operon:''' ''[[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed] |
− | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1709162 PubMed] |
* '''Regulation:''' | * '''Regulation:''' | ||
+ | ** induced in the absence of uridine nucleotides ([[PyrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed] | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
+ | ** [[PyrR]]: [[RNA switch]], transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) [http://www.ncbi.nlm.nih.gov/sites/entrez/8955403 PubMed] | ||
− | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = |
Revision as of 20:09, 8 June 2009
- Description: carbamoyl-phosphate synthetase (catalytic subunit)
Gene name | pyrAB |
Synonyms | |
Essential | no |
Product | carbamoyl-phosphate synthetase (catalytic subunit) |
Function | pyrimidine biosynthesis |
MW, pI | 117 kDa, 4.785 |
Gene length, protein length | 3213 bp, 1071 aa |
Immediate neighbours | pyrAA, pyrK |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU15520
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
- Protein family: carB family (according to Swiss-Prot)
- Paralogous protein(s): CarB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: membrane PubMed
Database entries
- Structure:
- Swiss prot entry: P25994
- KEGG entry: [3]
- E.C. number: 6.3.5.5
Additional information
Expression and regulation
- Regulatory mechanism:
- PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)