DegU

• Description: two-component response regulator, regulation of degradative enzyme and genetic competence, involved in activation of capsule biosynthetic operon expression (together with SwrAA), non-phosphorylated DegU is required for swarming motility PubMed
  
  

Gene name	degU
Synonyms	sacU, iep
Essential	no
Product	two-component response regulator
Function	regulation of degradative enzyme and genetic competence of genetic competence
MW, pI	25 kDa, 5.602
Gene length, protein length	687 bp, 229 aa
Immediate neighbours	yviA, degS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activator of degradative enzyme genes (sacB and degQ) and of comK expression

• Protein family:

• Paralogous protein(s):

Genes controlled by DegU

• Activation by DegU-P: degS-degU

• Activation by DegU: epr PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DegU-RapG, DegS-DegU

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P13800

• KEGG entry: BSU35490

• E.C. number:

Expression and regulation

• Operon: degS-degU

• Sigma factor: SigA

• Regulation: induced in the presence of ammonium (TnrA)

• Regulatory mechanism: TnrA: repression, DegU: positive autoregulation

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Monica Gupta, Kestur Krishnamurthy Rao
Epr plays a key role in DegU-mediated swarming motility of Bacillus subtilis.
FEMS Microbiol Lett: 2009, 295(2);187-94
[PubMed:19416356] [WorldCat.org] [DOI] (I p)

Cecilia Osera, Giuseppe Amati, Cinzia Calvio, Alessandro Galizzi
SwrAA activates poly-gamma-glutamate synthesis in addition to swarming in Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 7);2282-2287
[PubMed:19389763] [WorldCat.org] [DOI] (P p)

Ewan J Murray, Taryn B Kiley, Nicola R Stanley-Wall
A pivotal role for the response regulator DegU in controlling multicellular behaviour.
Microbiology (Reading): 2009, 155(Pt 1);1-8
[PubMed:19118340] [WorldCat.org] [DOI] (P p)