Difference between revisions of "CysS"
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot) |
* '''Protein family:''' class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot) | * '''Protein family:''' class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot) | ||
Revision as of 10:29, 23 May 2009
- Description: cysteine-tRNA synthetase
| Gene name | cysS |
| Synonyms | snpA |
| Essential | yes PubMed |
| Product | cysteine-tRNA synthetase |
| Function | translation |
| MW, pI | 53 kDa, 5.07 |
| Gene length, protein length | 1398 bp, 466 aa |
| Immediate neighbours | cysE, mrnC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on Ser-270 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: Q06752
- KEGG entry: BSU00940
- E.C. number: 6.1.1.16
Additional information
Expression and regulation
- Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
- Gagnon Y., Breton R., Putzer H., Pelchat M., Grunberg-Manago M., Lapointe J. (1994) Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis. J. Biol. Chem. 269: 7473-7482. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
