Difference between revisions of "OpuAC"
| Line 76: | Line 76: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CHG 3CHG] (in complex with DMSA) | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2B4L 2B4L] (complex with glycine betaine), [http://www.rcsb.org/pdb/explore.do?structureId=3CHG 3CHG] (in complex with DMSA) |
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P46922 P46922] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P46922 P46922] | ||
Revision as of 22:02, 5 May 2009
- Description: glycine betaine ABC transporter (binding protein)
| Gene name | opuAC |
| Synonyms | |
| Essential | no |
| Product | glycine betaine ABC transporter (binding protein) |
| Function | compatible solute transport |
| MW, pI | 32 kDa, 8.015 |
| Gene length, protein length | 879 bp, 293 aa |
| Immediate neighbours | opuAB, amhX |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Swiss prot entry: P46922
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany homepage
Your additional remarks
References
- Smits SH, Höing M, Lecher J, Jebbar M, Schmitt L, Bremer E. (2008) The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies. J Bacteriol. Aug;190(16): 5663-71. PubMed
- Kempf B, Bremer E. (1995) OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis. J Biol Chem. Jul 14;270(28): 16701-13. PubMed
- Horn C, Bremer E, Schmitt L. (2005) Functional overexpression and in vitro re-association of OpuA, an osmotically regulated ABC-transport complex from Bacillus subtilis. FEBS Lett. Oct 24;579(25): 5765-8. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
