Difference between revisions of "Tkt"
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | * '''Expression vector:''' | + | * '''Expression vector:''' pGP94 (N-terminal Strep-tag, for [[SPINE]], expression in B. subtilis, in [[pGP380]]), available in [[Stülke]] lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
Revision as of 10:45, 6 May 2009
- Description: transketolase
| Gene name | tkt |
| Synonyms | tktA |
| Essential | no |
| Product | transketolase |
| Function | pentose phosphate pathway |
| MW, pI | 72 kDa, 4.803 |
| Gene length, protein length | 2001 bp, 667 aa |
| Immediate neighbours | ynzC, yneE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylated on ser/ thr/ tyr PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P45694
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
