MtlA

• Description: trigger enzyme: mannitol-specific phosphotransferase system, EIICBA
  
  

Gene name	mtlA
Synonyms
Essential	no
Product	trigger enzyme: mannitol-specific phosphotransferase system, EIICBA
Function	mannitol uptake and phosphorylation, control of MtlR activity
MW, pI	65 kDa, 8.778
Gene length, protein length	1830 bp, 610 aa
Immediate neighbours	ycnL, mtlD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-559 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MtlA-MtlR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P42956

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: mtlA-mtlD

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
2. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed