Difference between revisions of "CcpN"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' DB104 ''ccpN''::[[cat]], available in [[Sabine Brantl]] lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''Antibody:''' | + | * '''Antibody:''' available in [[Sabine Brantl]] lab |
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
Revision as of 16:00, 9 February 2009
- Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
| Gene name | ccpN |
| Synonyms | yqzB |
| Essential | no |
| Product | transcriptional regulator |
| Function | repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1 |
| MW, pI | 23.4 kDa, 7.22 |
| Gene length, protein length | 636 bp, 212 amino acids |
| Immediate neighbours | glyS, yqfL |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates: 2604195 - 2604830
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: [3]
- KEGG entry: [4]
Additional information
Expression and regulation
- Sigma factor:
- Regulation: constitutively expressed PubMed
- Additional information:
Biological materials
- Mutant: DB104 ccpN::cat, available in Sabine Brantl lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody: available in Sabine Brantl lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Your additional remarks
References
- Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. PubMed
- Licht et al. (2005) Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis. Mol. Microbiol. 58: 189-206. PubMed
- Licht & Brantl (2006) Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding. J. Mol. Biol. 364: 434-448. PubMed
- Licht et al. (2008) Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis. J. Mol. Biol. 380: 17-30. PubMed
- Tännler et al. (2008) CcpN controls central carbon fluxes in Bacillus subtilis. J. Bacteriol. 190: 6178-6187. PubMed
