SigI

• Description: RNA polymerase sigma factor SigI
  
  

Gene name	sigI
Synonyms	ykoZ
Essential	no
Product	RNA polymerase sigma factor SigI
Function	control of a class of heat shock genes
MW, pI	29 kDa, 8.306
Gene length, protein length	753 bp, 251 aa
Immediate neighbours	ykoY, rsgI
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

temperature-sensitive growth on agar plates PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Members of the SigI regulon

gsiB PubMed, mreBH, bcrC PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RsgI-SigI, the interaction inhibits SigI activity PubMed

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
• Operon: sigI-rsgI PubMed

• Sigma factor: SigI PubMed

• Regulation: induced by heat PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y. (2007) Regulatory role of RsgI in sigI expression in Bacillus subtilis. Microbiology 153: 92-101. PubMed
2. Zuber, U., K. Drzewiecki, and M. Hecker (2001) Putative sigma factor SigI (YkoZ) of Bacillus subtilis is induced by heat shock J. Bacteriol. 183: 1472-1475. PubMed
3. Tseng, C. L., and Shaw, G. C. (2008) Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor SigI of Bacillus subtilis. J. Bacteriol. 190: 1561-1567. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed