Difference between revisions of "PAC7"

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[[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']]
 
[[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']]
 +
[[File:PAC7_cloning_region.jpeg|250px|thumb|right|'''pAC7 cloning region: click to enlarge''']]
  
 
pAC7
 
pAC7
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similar vectors: [[pAC5]], [[pAC6]]
 
similar vectors: [[pAC5]], [[pAC6]]
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 +
sequencing primer:
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*'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’
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*'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘
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*'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
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Weinrauch et al. (1991) J Bacteriol.  173:5685-5693. [http://www.ncbi.nlm.nih.gov/sites/entrez/1715859  PubMed]
 
Weinrauch et al. (1991) J Bacteriol.  173:5685-5693. [http://www.ncbi.nlm.nih.gov/sites/entrez/1715859  PubMed]

Revision as of 12:03, 18 March 2009

pAC7: click to enlarge
pAC7 cloning region: click to enlarge

pAC7

vector for the construction of translational lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: kanamycin resistance

similar vectors: pAC5, pAC6

sequencing primer:

  • PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
  • PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
  • Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘


Weinrauch et al. (1991) J Bacteriol. 173:5685-5693. PubMed