Difference between revisions of "PGP3299"
(Created page with "N-term STRP Expression vector for high level protein production with B. subtilis. Expression of the gene of interest will be under control of the IPTG inducible Pgrac promoter...") |
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| − | + | C-term 6xHIS tag Expression vector for high level protein production with B. subtilis. Expression of the gene of interest will be under control of the IPTG inducible Pgrac promoter. E.coli / B. subtilis shuttle vector (amp/cm) | |
sequencing primers: | sequencing primers: | ||
*'''CB242:''' 5’-AGCTAACGGAAAAGGGAGCG-3’ | *'''CB242:''' 5’-AGCTAACGGAAAAGGGAGCG-3’ | ||
*'''CB243:''' 5’-CATACGAGGCTAATATCGGCTTAT-3‘ | *'''CB243:''' 5’-CATACGAGGCTAATATCGGCTTAT-3‘ | ||
Latest revision as of 09:08, 26 June 2019
C-term 6xHIS tag Expression vector for high level protein production with B. subtilis. Expression of the gene of interest will be under control of the IPTG inducible Pgrac promoter. E.coli / B. subtilis shuttle vector (amp/cm)
sequencing primers:
- CB242: 5’-AGCTAACGGAAAAGGGAGCG-3’
- CB243: 5’-CATACGAGGCTAATATCGGCTTAT-3‘
