Difference between revisions of "HemAT"
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* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
** forms clusters at the cell poles {{PubMed|21515776}} | ** forms clusters at the cell poles {{PubMed|21515776}} | ||
− | ** homogeneous | + | ** homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase {{PubMed|23180473}} |
** polar foci in individual cells at later growth stages {{PubMed|23180473}} | ** polar foci in individual cells at later growth stages {{PubMed|23180473}} | ||
Revision as of 14:49, 3 July 2015
- Description: soluble chemotaxis receptor, heme-containing O(2) sensor protein
Gene name | hemAT |
Synonyms | yhfV |
Essential | no |
Product | haem-based aerotactic transducer |
Function | movement towards oxygen |
Gene expression levels in SubtiExpress: hemAT | |
Interactions involving this protein in SubtInteract: HemAT | |
MW, pI | 48 kDa, 5.441 |
Gene length, protein length | 1296 bp, 432 aa |
Immediate neighbours | yhfU, yhfW |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10380
Phenotypes of a mutant
- not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
- NCIB3610 hemAT mutant has higher fitness than hag mutant during pellicle formation PubMed
- NCIB3610 hemAT-hag double mutant has similar fitness to single hag mutant during pellicle formation PubMed
Database entries
- BsubCyc: BSU10380
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
- HemAT protein has a moderate oxygen affinity PubMed
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): heme
- Effectors of protein activity:
Database entries
- BsubCyc: BSU10380
- UniProt: O07621
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: hemAT PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
- in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 1095 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 1803 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3169 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 966 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1236 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Markéta Martínková, Kenichi Kitanishi, Toru Shimizu
Heme-based globin-coupled oxygen sensors: linking oxygen binding to functional regulation of diguanylate cyclase, histidine kinase, and methyl-accepting chemotaxis.
J Biol Chem: 2013, 288(39);27702-11
[PubMed:23928310]
[WorldCat.org]
[DOI]
(I p)
Original publications