YcgT

• Description: NADPH:ferredoxin oxidoreductase
  
  

Gene name	ycgT
Synonyms
Essential	no
Product	NADPH:ferredoxin oxidoreductase
Function	unknown
Gene expression levels in SubtiExpress: ycgT
MW, pI	36 kDa, 5.61
Gene length, protein length	1008 bp, 336 aa
Immediate neighbours	ycgS, nasF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other

This gene is a member of the following regulons

Fur regulon

The gene

Basic information

• Locus tag: BSU03270

Database entries

• BsubCyc: BSU03270

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 reduced ferredoxin + NADP⁺ + H⁺ = 2 oxidized ferredoxin + NADPH (according to Swiss-Prot)

• Protein family: ferredoxin--NADP reductase type 2 family (according to Swiss-Prot)

• Paralogous protein(s): YumC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors: FAD PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03270

• Structure:

• UniProt: O31475

• KEGG entry: [3]

• E.C. number:

Additional information

The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. PubMed

Expression and regulation

• Operon: ycgT (according to DBTBS)

• Expression browser: ycgT PubMed

• Sigma factor:

• Regulation: induced under conditions of iron starvation (Fur) PubMed

• Regulatory mechanism: Fur: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1152 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 572 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 617 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 256 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 84 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Andreas Mielcarek, Bastian Blauenburg, Marcus Miethke, Mohamed A Marahiel
Molecular insights into frataxin-mediated iron supply for heme biosynthesis in Bacillus subtilis.
PLoS One: 2015, 10(3);e0122538
[PubMed:25826316] [WorldCat.org] [DOI] (I e)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)