Difference between revisions of "SwrAA/1"
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| − | * '''Description:''' modulator of [[DegU]] activity, converts [[DegU]]-P from a repressor to an activator of the | + | * '''Description:''' modulator of [[DegU]] activity, converts [[DegU]]-P from a repressor to an activator of the [[fla/che operon]], enhances ''[[sigD]]'' transcription, controls the number of flagellar basal bodies, inactive pseudogene in strain 168 <br/><br/> |
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* This protein is functional in undomesticated strains of ''B.subtilis'' but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of ''B.subtilis'' are unable to swarm. | * This protein is functional in undomesticated strains of ''B.subtilis'' but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of ''B.subtilis'' are unable to swarm. | ||
* The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of ''swrAA'' was predicted to be an ORF (''[[yvzD]]'') by the genome project. | * The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of ''swrAA'' was predicted to be an ORF (''[[yvzD]]'') by the genome project. | ||
| − | * Correction of ''[[sfp]]'', ''[[epsC]]'','' [[swrAA]]'', and ''[[degQ]]'' as well as introduction of ''rapP'' from a plasmid present in NCIB3610 results in biofilm formation in ''B. subtilis'' 168 {{PubMed|21278284}} | + | * Correction of ''[[sfp]]'', ''[[epsC]]'','' [[swrAA]]'', and ''[[degQ]]'' as well as introduction of ''rapP'' from a plasmid present in NCIB3610 results in [[biofilm formation]] in ''B. subtilis'' 168 {{PubMed|21278284}} |
=The protein= | =The protein= | ||
| Line 150: | Line 150: | ||
<pubmed>20735481 22092493 </pubmed> | <pubmed>20735481 22092493 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>16091050 22773650 16357223,19389763,16030230,18567663,15066026 22496484 19389763 19749039 21278284 21602220,22329926,23190039 24386445 12864845 25538299</pubmed> | + | <pubmed>16091050 22773650 16357223,19389763,16030230,18567663,15066026 22496484 19389763 19749039 21278284 21602220,22329926,23190039 24386445 12864845 25538299 25843804</pubmed> |
[[Category:Pseudogenes]] | [[Category:Pseudogenes]] | ||
Revision as of 08:03, 8 April 2015
- Description: modulator of DegU activity, converts DegU-P from a repressor to an activator of the fla/che operon, enhances sigD transcription, controls the number of flagellar basal bodies, inactive pseudogene in strain 168
| Gene name | swrAA/1 |
| Synonyms | yvzD, swrAA, ifm |
| Essential | no |
| Product | swarming motility protein |
| Function | control of DegU activity |
| Gene expression levels in SubtiExpress: swrAA/1 | |
| Interactions involving this protein in SubtInteract: SwrA | |
| MW, pI | - , - |
| Gene length, protein length | 336 bp, - |
| Immediate neighbours | minJ, swrAA/2 |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biofilm formation, motility and chemotaxis, transcription factors and their control, pseudogenes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35230
Phenotypes of a mutant
- loss of swarming motility PubMed
Database entries
- BsubCyc: BSU35230
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
- This protein is functional in undomesticated strains of B.subtilis but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of B.subtilis are unable to swarm.
- The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of swrAA was predicted to be an ORF (yvzD) by the genome project.
- Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU35230
- Structure:
- Swiss prot entry: O32266
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Joyce E Patrick, Daniel B Kearns
Swarming motility and the control of master regulators of flagellar biosynthesis.
Mol Microbiol: 2012, 83(1);14-23
[PubMed:22092493]
[WorldCat.org]
[DOI]
(I p)
Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481]
[WorldCat.org]
[DOI]
(I p)
Original publications
Yuichi Hakumai, Kouko Shimomoto, Makoto Ashiuchi
Extra-chromosomal DNA maintenance in Bacillus subtilis, dependence on flagellation factor FliF and moonlighting mediator EdmS.
Biochem Biophys Res Commun: 2015, 460(4);1059-62
[PubMed:25843804]
[WorldCat.org]
[DOI]
(I p)
Sampriti Mukherjee, Anna C Bree, Jing Liu, Joyce E Patrick, Peter Chien, Daniel B Kearns
Adaptor-mediated Lon proteolysis restricts Bacillus subtilis hyperflagellation.
Proc Natl Acad Sci U S A: 2015, 112(1);250-5
[PubMed:25538299]
[WorldCat.org]
[DOI]
(I p)
Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445]
[WorldCat.org]
[DOI]
(I e)
Sarah B Guttenplan, Sidney Shaw, Daniel B Kearns
The cell biology of peritrichous flagella in Bacillus subtilis.
Mol Microbiol: 2013, 87(1);211-29
[PubMed:23190039]
[WorldCat.org]
[DOI]
(I p)
Emilia Ghelardi, Sara Salvetti, Mara Ceragioli, Sokhna A Gueye, Francesco Celandroni, Sonia Senesi
Contribution of surfactin and SwrA to flagellin expression, swimming, and surface motility in Bacillus subtilis.
Appl Environ Microbiol: 2012, 78(18);6540-4
[PubMed:22773650]
[WorldCat.org]
[DOI]
(I p)
Mitsuo Ogura, Kensuke Tsukahara
SwrA regulates assembly of Bacillus subtilis DegU via its interaction with N-terminal domain of DegU.
J Biochem: 2012, 151(6);643-55
[PubMed:22496484]
[WorldCat.org]
[DOI]
(I p)
Loralyn M Cozy, Andrew M Phillips, Rebecca A Calvo, Ashley R Bate, Yi-Huang Hsueh, Richard Bonneau, Patrick Eichenberger, Daniel B Kearns
SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.
Mol Microbiol: 2012, 83(6);1210-28
[PubMed:22329926]
[WorldCat.org]
[DOI]
(I p)
Kassem Hamze, Sabine Autret, Krzysztof Hinc, Soumaya Laalami, Daria Julkowska, Romain Briandet, Margareth Renault, Cédric Absalon, I Barry Holland, Harald Putzer, Simone J Séror
Single-cell analysis in situ in a Bacillus subtilis swarming community identifies distinct spatially separated subpopulations differentially expressing hag (flagellin), including specialized swarmers.
Microbiology (Reading): 2011, 157(Pt 9);2456-2469
[PubMed:21602220]
[WorldCat.org]
[DOI]
(I p)
Anna L McLoon, Sarah B Guttenplan, Daniel B Kearns, Roberto Kolter, Richard Losick
Tracing the domestication of a biofilm-forming bacterium.
J Bacteriol: 2011, 193(8);2027-34
[PubMed:21278284]
[WorldCat.org]
[DOI]
(I p)
Joyce E Patrick, Daniel B Kearns
Laboratory strains of Bacillus subtilis do not exhibit swarming motility.
J Bacteriol: 2009, 191(22);7129-33
[PubMed:19749039]
[WorldCat.org]
[DOI]
(I p)
Cecilia Osera, Giuseppe Amati, Cinzia Calvio, Alessandro Galizzi
SwrAA activates poly-gamma-glutamate synthesis in addition to swarming in Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 7);2282-2287
[PubMed:19389763]
[WorldCat.org]
[DOI]
(P p)
Cinzia Calvio, Cecilia Osera, Giuseppe Amati, Alessandro Galizzi
Autoregulation of swrAA and motility in Bacillus subtilis.
J Bacteriol: 2008, 190(16);5720-8
[PubMed:18567663]
[WorldCat.org]
[DOI]
(I p)
Daniel B Kearns, Richard Losick
Cell population heterogeneity during growth of Bacillus subtilis.
Genes Dev: 2005, 19(24);3083-94
[PubMed:16357223]
[WorldCat.org]
[DOI]
(P p)
Nicola R Stanley, Beth A Lazazzera
Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly-gamma-dl-glutamic acid production and biofilm formation.
Mol Microbiol: 2005, 57(4);1143-58
[PubMed:16091050]
[WorldCat.org]
[DOI]
(P p)
Cinzia Calvio, Francesco Celandroni, Emilia Ghelardi, Giuseppe Amati, Sara Salvetti, Fabrizio Ceciliani, Alessandro Galizzi, Sonia Senesi
Swarming differentiation and swimming motility in Bacillus subtilis are controlled by swrA, a newly identified dicistronic operon.
J Bacteriol: 2005, 187(15);5356-66
[PubMed:16030230]
[WorldCat.org]
[DOI]
(P p)
Daniel B Kearns, Frances Chu, Rivka Rudner, Richard Losick
Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motility.
Mol Microbiol: 2004, 52(2);357-69
[PubMed:15066026]
[WorldCat.org]
[DOI]
(P p)
Daniel B Kearns, Richard Losick
Swarming motility in undomesticated Bacillus subtilis.
Mol Microbiol: 2003, 49(3);581-90
[PubMed:12864845]
[WorldCat.org]
[DOI]
(P p)
