Difference between revisions of "PhoR"

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(References)
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
 
* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
  
* '''Modification:''' autophosphorylation on a His residue  
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* '''Modification:''' autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [[PAS domain]] of [[PhoR]] {{PubMed 25315493}}
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''  
 
* '''Effectors of protein activity:'''  
  
* '''[[SubtInteract|Interactions]]:''' [[PhoP]]-[[PhoR]]
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* '''[[SubtInteract|Interactions]]:'''  
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** [[PhoP]]-[[PhoR]]
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29100&redirect=T BSU29100]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29100&redirect=T BSU29100]
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CWF 3CWF] (extracellular PAS domain)  {{PubMed|20008068}}  
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3CWF 3CWF] (extracellular domain)  {{PubMed|20008068}}  
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P23545 P23545]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P23545 P23545]
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=References=
 
=References=
  
<pubmed>20008068 10433720,15205429,9084179,17085571,9987123,10913081,10094672,16452408 20167622 ,15205429,14762014, 20382764 25315493 </pubmed>
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<pubmed>20008068 10433720,15205429,9084179,17085571,9987123,10913081,10094672,16452408 20167622 ,15205429,14762014, 20382764 25315493</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:55, 27 October 2014

  • Description: two-component sensor kinase, regulation of phosphate metabolism

Gene name phoR
Synonyms
Essential no
Product two-component sensor kinase
Function regulation of phosphate metabolism
Gene expression levels in SubtiExpress: phoR
Interactions involving this protein in SubtInteract: PhoR
MW, pI 64 kDa, 5.957
Gene length, protein length 1737 bp, 579 aa
Immediate neighbours polA, phoP
Sequences Protein DNA DNA_with_flanks
Genetic context
PhoR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PhoR expression.png















Categories containing this gene/protein

phosphate metabolism, protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, SigB regulon, SigE regulon

The gene

Basic information

  • Locus tag: BSU29100

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of PhoP
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification: autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular PAS domain of PhoR Template:PubMed 25315493
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • carbon catabolite repression (CcpA) PubMed
    • expressed under conditions of phosphate limitation (PhoP) PubMed
    • expressed in post-exponential phase (ScoC) PubMed
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 453 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

Your additional remarks

References