MurG

• Description: UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II
  
  

Gene name	murG
Synonyms
Essential	yes PubMed
Product	UDP-N-acetylglucosamine- N-acetylmuramyl-(pentapeptide)pyrophosphoryl- undecaprenol N-acetylglucosamine transferase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murG
Metabolic function and regulation of this protein in SubtiPathways: murG
MW, pI	39 kDa, 9.568
Gene length, protein length	1089 bp, 363 aa
Immediate neighbours	spoVE, murB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, sporulation proteins, essential genes, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU15220

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15220

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide molecule
  • UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)

• Protein family: MurG subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • peripheral membrane protein PubMed

Database entries

• BsubCyc: BSU15220

• Structure: 1F0K (from Escherichia coli, 30% identity, 52% similarity) PubMed

• UniProt: P37585

• KEGG entry: [3]

• E.C. number: 2.4.1.227

Expression and regulation

• Operon:
  • murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • murG-murB-divIB-ylxW-ylxX-sbp PubMed

• Expression browser: murG PubMed

• Sigma factor:
  • spoVE: SigE PubMed
  • within murG: SigM PubMed

• Regulation:
  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 82 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 627 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct:
  • GP2002 murG-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab

• Antibody: