PrkA

• Description: putative serine protein kinase
  
  

Gene name	prkA
Synonyms
Essential	no
Product	putative serine protein kinase
Function	unknown
Gene expression levels in SubtiExpress: prkA
MW, pI	72 kDa, 5.459
Gene length, protein length	1893 bp, 631 aa
Immediate neighbours	yhbF, yhbH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU08970

Phenotypes of a mutant

sporulation frequency reduced to 12-27% of wild-type levels PubMed

Database entries

• BsubCyc: BSU08970

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: prkA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU08970

• Structure:

• UniProt: P39134

• KEGG entry: [3]

• E.C. number:

Additional information

• the corresponding protein from E. coli (YeaG)

Expression and regulation

• Operon: prkA PubMed

• Expression browser: prkA PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 34 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 170 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 22 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 482 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jihen Tagourti, Ahmed Landoulsi, Gilbert Richarme
Cloning, expression, purification and characterization of the stress kinase YeaG from Escherichia coli.
Protein Expr Purif: 2008, 59(1);79-85
[PubMed:18276156] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

C Fischer, C Geourjon, C Bourson, J Deutscher
Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase.
Gene: 1996, 168(1);55-60
[PubMed:8626065] [WorldCat.org] [DOI] (P p)