Adk

• Description: adenylate kinase
  
  

Gene name	adk
Synonyms
Essential	yes PubMed
Product	adenylate kinase
Function	ADP formation
Gene expression levels in SubtiExpress: adk
Metabolic function and regulation of this protein in SubtiPathways: adk
MW, pI	23 kDa, 4.449
Gene length, protein length	651 bp, 217 aa
Immediate neighbours	secY, map
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01370

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01370

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + AMP = 2 ADP (according to Swiss-Prot)

• Protein family: adenylate kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU01370

• Structure: 1P3J

• UniProt: P16304

• KEGG entry: [2]

• E.C. number: 2.7.4.3 2.7.4.3]

Expression and regulation

• Operon:

• Expression browser: adk PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1470 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 866 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1827 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thomas H Segall-Shapiro, Peter Q Nguyen, Edgardo D Dos Santos, Saurav Subedi, Justin Judd, Junghae Suh, Jonathan J Silberg
Mesophilic and hyperthermophilic adenylate kinases differ in their tolerance to random fragmentation.
J Mol Biol: 2011, 406(1);135-48
[PubMed:21145325] [WorldCat.org] [DOI] (I p)

Rafael Couñago, Corey J Wilson, Matthew I Peña, Pernilla Wittung-Stafshede, Yousif Shamoo
An adaptive mutation in adenylate kinase that increases organismal fitness is linked to stability-activity trade-offs.
Protein Eng Des Sel: 2008, 21(1);19-27
[PubMed:18093993] [WorldCat.org] [DOI] (P p)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)