AcsA

• Description: acetyl-CoA synthetase
  
  

Gene name	acsA
Synonyms
Essential	no
Product	acetyl-CoA synthetase)
Function	utilization of acetate, fatty acids
Gene expression levels in SubtiExpress: acsA
Metabolic function and regulation of this protein in SubtiPathways: acsA
MW, pI	64 kDa, 5.547
Gene length, protein length	1716 bp, 572 aa
Immediate neighbours	ytzK, acuA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

CcpA regulon, CodY regulon

The gene

Basic information

• Locus tag: BSU29680

Database entries

• BsubCyc: BSU29680

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29680

• Structure:

• UniProt: P39062

• KEGG entry: [3]

• E.C. number: 6.2.1.1

Expression and regulation

• Operon: acsA PubMed

• Expression browser: acsA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (4.5-fold) (CcpA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 467 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3357 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1384 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3490 PubMed

Biological materials

• Mutant:
  • GP1212 (acsA::kan), available in Stülke lab
• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Eric L Hegg
Unraveling the structure and mechanism of acetyl-coenzyme A synthase.
Acc Chem Res: 2004, 37(10);775-83
[PubMed:15491124] [WorldCat.org] [DOI] (P p)

V J Starai, J C Escalante-Semerena
Acetyl-coenzyme A synthetase (AMP forming).
Cell Mol Life Sci: 2004, 61(16);2020-30
[PubMed:15316652] [WorldCat.org] [DOI] (P p)

Paul A Lindahl
Acetyl-coenzyme A synthase: the case for a Ni(p)(0)-based mechanism of catalysis.
J Biol Inorg Chem: 2004, 9(5);516-24
[PubMed:15221478] [WorldCat.org] [DOI] (P p)