RbgA

• Description: assembly of the 50S subunit of the ribosome
  
  

Gene name	rbgA
Synonyms	ylqF
Essential	yes PubMed
Product	GTPase
Function	ribosome assembly
Gene expression levels in SubtiExpress: rbgA
Interactions involving this protein in SubtInteract: RbgA
MW, pI	31 kDa, 9.528
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	rplS, rnhB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

The gene

Basic information

• Locus tag: BSU16050

Phenotypes of a mutant

• essential PubMed
• depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
• premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including L16, L27, L28, L33, L35 and L36 PubMed

Database entries

• BsubCyc: BSU16050

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • proper positioning of L6 in the large subunit of the ribosome prior to incorporation of L16 PubMed
  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:
  • RNA-binding domain at the C-terminus PubMed
• Modification:

• Cofactors:

• Effectors of protein activity:
  • GTPase activity requires binding of L16 to the 50S subunit of the ribosome PubMed

• Interactions:
  • RbgA-RplF PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16050

• Structure: 1PUJ

• UniProt: O31743

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: rbgA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 94 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 568 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan