Difference between revisions of "YtdI"

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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[RoxS]]: inhibition of translation and initiation of RNA degradation by [[rny|RNase Y]] and [[rnc|RNase III]] {{PubMed|25643072}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
 +
** the mRNA base-pairs with the [[RoxS]] [[sRNA]] {{PubMed|25643072}}
 +
** half-life of the mRNA: 8.4 min, in a ''[[roxS]]'' mutant 15 min, in a ''[[rny]]'' mutant 24 min {{PubMed|25643072}}
 
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 +
** two pathways of mRNA degradation: (i) by [[rny|RNase Y]], (ii) by combined action of [[RoxS]] and [[rnc|RNase III]] {{PubMed|25643072}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 714 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 714 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1384 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1384 {{PubMed|24696501}}
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=References=
 
=References=
<pubmed>16511117 21815947</pubmed>  
+
<pubmed>16511117 25643072 21815947</pubmed>  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:45, 4 February 2015

  • Description: inorganic polyphosphate/ATP-NAD kinase

Gene name ytdI
Synonyms ppnKB
Essential no
Product inorganic polyphosphate/ATP-NAD kinase
Function generation of NADP from NAD
Gene expression levels in SubtiExpress: ytdI
Metabolic function and regulation of this protein in SubtiPathways:
YtdI
MW, pI 30 kDa, 6.49
Gene length, protein length 801 bp, 267 aa
Immediate neighbours sppA, ytcJ
Sequences Protein DNA DNA_with_flanks
Genetic context
YtdI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YtdI expression.png















Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + NAD+ = ADP + NADP+ (according to Swiss-Prot)
  • Protein family: NAD kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1YT5 (from Thermotoga maritima) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Additional information:
    • the mRNA base-pairs with the RoxS sRNA PubMed
    • half-life of the mRNA: 8.4 min, in a roxS mutant 15 min, in a rny mutant 24 min PubMed
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
    • two pathways of mRNA degradation: (i) by RNase Y, (ii) by combined action of RoxS and RNase III PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 714 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1384 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References