DltA

• Description: D-alanyl-D-alanine carrier protein ligase, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides
  
  

Gene name	dltA
Synonyms	ipa-5r, dae
Essential	no
Product	D-alanyl-D-alanine carrier protein ligase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: dltA
Metabolic function and regulation of this protein in SubtiPathways: DltA
MW, pI	55 kDa, 4.929
Gene length, protein length	1509 bp, 503 aa
Immediate neighbours	ywzH, dltB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigD regulon, SigM regulon, SigX regulon, Spo0A regulon, stringent response, YvrHb regulon

The gene

Basic information

• Locus tag: BSU38500

Phenotypes of a mutant

• • increased sensitivity to lysozyme PubMed
• more sensitive to nisin PubMed

Database entries

• BsubCyc: BSU38500

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) (according to Swiss-Prot)

• Protein family: DltA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU38500

• Structure: 3E7X

• UniProt: P39581

• KEGG entry: [3]

• E.C. number: 6.1.1.13

Expression and regulation

• Operon: dltA-dltB-dltC-dltD-dltE PubMed

• Expression browser: dltA PubMed

• Sigma factor: SigD PubMed, SigM PubMed, SigX PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • activated by YvrHb PubMed
  • Spo0A: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1023 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2317 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage