IspH

• Description: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispH
Synonyms	yqfP
Essential	yes PubMed
Product	(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispH
Metabolic function and regulation of this protein in SubtiPathways: IspH
MW, pI	34 kDa, 5.615
Gene length, protein length	942 bp, 314 aa
Immediate neighbours	yqfQ, yqfO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

The gene

Basic information

• Locus tag: BSU25160

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25160

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isopentenyl diphosphate + NAD(P)⁺ + H₂O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H (according to Swiss-Prot)

• Protein family: ispH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU25160

• Structure: 3F7T (IspH from E. coli, 38% identity) PubMed

• UniProt: P54473

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ispH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 175 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 433 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Tobias Gräwert, Felix Rohdich, Ingrid Span, Adelbert Bacher, Wolfgang Eisenreich, Jörg Eppinger, Michael Groll
Structure of active IspH enzyme from Escherichia coli provides mechanistic insights into substrate reduction.
Angew Chem Int Ed Engl: 2009, 48(31);5756-9
[PubMed:19569147] [WorldCat.org] [DOI] (I p)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)