Difference between revisions of "NrgA"
| Line 39: | Line 39: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | + | <br/><br/> | |
| − | |||
| − | |||
| − | |||
| − | |||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| Line 69: | Line 65: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
| Line 87: | Line 80: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 118: | Line 111: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=amtB_3756790_3758004_1 nrgA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=amtB_3756790_3758004_1 nrgA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|8282685}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8282685}} |
* '''Regulation:''' induced by ammonium limitation | * '''Regulation:''' induced by ammonium limitation | ||
| Line 130: | Line 123: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' GP739 (cat), GP255 ('' | + | * '''Mutant:''' GP739 (cat), GP255 (''[[nrgA]]-[[nrgB]]'', cat), both available in [[Jörg Stülke]]'s lab {{PubMed|14600241}} |
** 1A920 ( ''nrgA''::''erm''), {{PubMed|8282685}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A920&Search=1A920 BGSC] | ** 1A920 ( ''nrgA''::''erm''), {{PubMed|8282685}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A920&Search=1A920 BGSC] | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | * '''lacZ fusion:''' pGP168 (in [[pAC7]]), available in [[Stülke]] lab | + | * '''lacZ fusion:''' pGP168 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab {{PubMed|14600241}} |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
Revision as of 12:17, 21 May 2014
- Description: ammonium transporter, required at low ammonium concentration
| Gene name | nrgA |
| Synonyms | amtB |
| Essential | no |
| Product | ammonium transporter |
| Function | ammonium uptake |
| Gene expression levels in SubtiExpress: nrgA | |
| Interactions involving this protein in SubtInteract: NrgA | |
| Metabolic function and regulation of this protein in SubtiPathways: nrgA | |
| MW, pI | 42 kDa, 5.727 |
| Gene length, protein length | 1212 bp, 404 aa |
| Immediate neighbours | ywoB, nrgB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transporters/ other, biosynthesis/ acquisition of amino acids, glutamate metabolism, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36510
Phenotypes of a mutant
Database entries
- BsubCyc: BSU36510
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot), membrane
Database entries
- BsubCyc: BSU36510
- UniProt: Q07429
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: GP739 (cat), GP255 (nrgA-nrgB, cat), both available in Jörg Stülke's lab PubMed
- Expression vector:
- lacZ fusion: pGP168 (in pAC7), available in Jörg Stülke's lab PubMed
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
Reviews
Arnaud Javelle, Domenico Lupo, Xiao-Dan Li, Mike Merrick, Mohamed Chami, Pierre Ripoche, Fritz K Winkler
Structural and mechanistic aspects of Amt/Rh proteins.
J Struct Biol: 2007, 158(3);472-81
[PubMed:17368911]
[WorldCat.org]
[DOI]
(P p)
G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624]
[WorldCat.org]
[DOI]
(P p)
Original Publications
Matthew J Conroy, Anne Durand, Domenico Lupo, Xiao-Dan Li, Per A Bullough, Fritz K Winkler, Mike Merrick
The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.
Proc Natl Acad Sci U S A: 2007, 104(4);1213-8
[PubMed:17220269]
[WorldCat.org]
[DOI]
(P p)
Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076]
[WorldCat.org]
[DOI]
(P p)
Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading): 2003, 149(Pt 11);3289-3297
[PubMed:14600241]
[WorldCat.org]
[DOI]
(P p)
Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818]
[WorldCat.org]
[DOI]
(P p)
L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114]
[WorldCat.org]
[DOI]
(P p)
L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J Bacteriol: 1994, 176(1);108-14
[PubMed:8282685]
[WorldCat.org]
[DOI]
(P p)
M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935]
[WorldCat.org]
[DOI]
(P p)
