Mbl

• Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for CwlO activity
  
  

Gene name	mbl
Synonyms
Essential	no
Product	MreB-like protein
Function	cell shape determination
Gene expression levels in SubtiExpress: mbl
Interactions involving this protein in SubtInteract: Mbl
MW, pI	35 kDa, 5.669
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	flhO, spoIIID
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell shape, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, stringent response

The gene

Basic information

• Locus tag: BSU36410

Phenotypes of a mutant

• non-viable in the presence of low Mg(2+)
• readily accumulate rsgI suppressor mutants PubMed

Database entries

• BsubCyc: BSU36410

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • forms helical filaments in a heterologous system PubMed
  • required for CwlO activity PubMed

• Protein family: ftsA/mreB family (according to Swiss-Prot)

• Paralogous protein(s): MreB, MreBH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • part of the cell wall biosynthetic complex PubMed
  • MreB-Mbl PubMed
  • Mbl-MreBH PubMed
  • RodZ-Mbl PubMed

• Localization:
  • during logarithmic growth, Mbl forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • close to the inner surface of the cytoplasmic membrane PubMed
  • reports on helical structures formed by Mbl PubMed seem to be misinterpretation of data PubMed

Database entries

• BsubCyc: BSU36410

• Structure:

• UniProt: P39751

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: usd-spoIIID-mbl-flhO-flhP PubMed

• Expression browser: mbl PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) PubMed

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mbl PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 336 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2526 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody: available in the Jeff Errington and Peter Graumann labs

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Localization

Felix Dempwolff, Christian Reimold, Michael Reth, Peter L Graumann
Bacillus subtilis MreB orthologs self-organize into filamentous structures underneath the cell membrane in a heterologous cell system.
PLoS One: 2011, 6(11);e27035
[PubMed:22069484] [WorldCat.org] [DOI] (I p)

Ethan C Garner, Remi Bernard, Wenqin Wang, Xiaowei Zhuang, David Z Rudner, Tim Mitchison
Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis.
Science: 2011, 333(6039);222-5
[PubMed:21636745] [WorldCat.org] [DOI] (I p)

Julia Domínguez-Escobar, Arnaud Chastanet, Alvaro H Crevenna, Vincent Fromion, Roland Wedlich-Söldner, Rut Carballido-López
Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria.
Science: 2011, 333(6039);225-8
[PubMed:21636744] [WorldCat.org] [DOI] (I p)

Henrik Strahl, Leendert W Hamoen
Membrane potential is important for bacterial cell division.
Proc Natl Acad Sci U S A: 2010, 107(27);12281-6
[PubMed:20566861] [WorldCat.org] [DOI] (I p)

Hervé Joël Defeu Soufo, Peter L Graumann
Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the function of MreB.
Mol Microbiol: 2006, 62(5);1340-56
[PubMed:17064365] [WorldCat.org] [DOI] (P p)