CitG

• Description: fumarase
  
  

Gene name	citG
Synonyms
Essential	no
Product	fumarate hydratase
Function	TCA cycle
Gene expression levels in SubtiExpress: citG
Interactions involving this protein in SubtInteract: CitG
Metabolic function and regulation of this protein in SubtiPathways: citG
MW, pI	50 kDa, 5.475
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	yuxN, yvzF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, most abundant proteins

The gene

Basic information

• Locus tag: BSU33040

Database entries

• BsubCyc: BSU33040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate = fumarate + H₂O (according to Swiss-Prot)

• Protein family: Fumarase subfamily (according to Swiss-Prot)

• Paralogous protein(s): AnsB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • Mdh-CitG PubMed
  • CitG-CitB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU33040

• Structure: 3R6Q (aspartase from Bacillus sp. YM55-1, 43% identity, 77% similarity) PubMed

• UniProt: P07343

• KEGG entry: [3]

• E.C. number: 4.2.1.2

Expression and regulation

• Operon: citG PubMed

• Expression browser: citG PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2427 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 9880 PubMed

Biological materials

• Mutant:
  • GP718 (spec), available in Jörg Stülke's lab

• Expression vector:
  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)

• lacZ fusion:
  • pGP387 (in pAC7), available in Jörg Stülke's lab

• GFP fusion: GP1433 (spc, based on pGP1870), available in the Stülke lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

• FLAG-tag construct:
  • GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Guntur Fibriansah, Vinod Puthan Veetil, Gerrit J Poelarends, Andy-Mark W H Thunnissen
Structural basis for the catalytic mechanism of aspartate ammonia lyase.
Biochemistry: 2011, 50(27);6053-62
[PubMed:21661762] [WorldCat.org] [DOI] (I p)

Frederik M Meyer, Jan Gerwig, Elke Hammer, Christina Herzberg, Fabian M Commichau, Uwe Völker, Jörg Stülke
Physical interactions between tricarboxylic acid cycle enzymes in Bacillus subtilis: evidence for a metabolon.
Metab Eng: 2011, 13(1);18-27
[PubMed:20933603] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

V A Price, I M Feavers, A Moir
Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168.
J Bacteriol: 1989, 171(11);5933-9
[PubMed:2509423] [WorldCat.org] [DOI] (P p)

K M Tatti, H L Carter, A Moir, C P Moran
Sigma H-directed transcription of citG in Bacillus subtilis.
J Bacteriol: 1989, 171(11);5928-32
[PubMed:2509422] [WorldCat.org] [DOI] (P p)

I M Feavers, V Price, A Moir
The regulation of the fumarase (citG) gene of Bacillus subtilis 168.
Mol Gen Genet: 1988, 211(3);465-71
[PubMed:3130545] [WorldCat.org] [DOI] (P p)

J S Miles, J R Guest
Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.
Nucleic Acids Res: 1985, 13(1);131-40
[PubMed:3923430] [WorldCat.org] [DOI] (P p)