RsiX

• Description: anti-SigX
  
  

Gene name	rsiX
Synonyms	ypuN
Essential	no
Product	anti-SigX
Function	control of SigX activity
Gene expression levels in SubtiExpress: rsiX
Interactions involving this protein in SubtInteract: RsiX
MW, pI	41 kDa, 5.452
Gene length, protein length	1104 bp, 368 aa
Immediate neighbours	aroC, sigX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigX regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU23090

Phenotypes of a mutant

• inactivation of rsiX strongly restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• BsubCyc: BSU23090

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RsiX-SigX PubMed

• Localization: cell membrane

Database entries

• BsubCyc: BSU23090

• Structure:

• UniProt: P35166

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigX-rsiX PubMed

• Expression browser: rsiX PubMed

• Sigma factor: SigA PubMed, SigX PubMed

• Regulation:

• Regulatory mechanism: activated by YvrHb PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 108 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 90 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

Mika Yoshimura, Kei Asai, Yoshito Sadaie, Hirofumi Yoshikawa
Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors.
Microbiology (Reading): 2004, 150(Pt 3);591-599
[PubMed:14993308] [WorldCat.org] [DOI] (P p)

X Huang, J D Helmann
Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search.
J Mol Biol: 1998, 279(1);165-73
[PubMed:9636707] [WorldCat.org] [DOI] (P p)

S Brutsche, V Braun
SigX of Bacillus subtilis replaces the ECF sigma factor fecI of Escherichia coli and is inhibited by RsiX.
Mol Gen Genet: 1997, 256(4);416-25
[PubMed:9393439] [WorldCat.org] [DOI] (P p)

V Azevedo, A Sorokin, S D Ehrlich, P Serror
The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.
Mol Microbiol: 1993, 10(2);397-405
[PubMed:7934830] [WorldCat.org] [DOI] (P p)