YpwA

• Description: carboxypeptidase, metalloprotease
  
  

Gene name	ypwA
Synonyms
Essential	no
Product	M32 carboxypeptidase
Function	unknown
Gene expression levels in SubtiExpress: ypwA
MW, pI	58 kDa, 4.77
Gene length, protein length	1503 bp, 501 aa
Immediate neighbours	xpt, kdgT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis

The gene

Basic information

• Locus tag: BSU22080

Database entries

• BsubCyc: BSU22080

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: M32 carboxypeptidase

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • dimer PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU22080

• Structure: 3HQ2 PubMed

• UniProt: P50848

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ypwA (according to DBTBS)

• Expression browser: ypwA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 554 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1504 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marianne M Lee, Clara E Isaza, James D White, Rita P-Y Chen, George F-C Liang, Hannah T-F He, Sunney I Chan, Michael K Chan
Insight into the substrate length restriction of M32 carboxypeptidases: characterization of two distinct subfamilies.
Proteins: 2009, 77(3);647-57
[PubMed:19544567] [WorldCat.org] [DOI] (I p)