Tkt

• Description: transketolase
  
  

Gene name	tkt
Synonyms	tktA
Essential	no
Product	transketolase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: tkt
Interactions involving this protein in SubtInteract: Tkt
Metabolic function and regulation of this protein in SubtiPathways: tkt
MW, pI	72 kDa, 4.803
Gene length, protein length	2001 bp, 667 aa
Immediate neighbours	ynzC, sirA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU17890

Database entries

• BsubCyc: BSU17890

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)

• Protein family: transketolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactors: thiamine pyrophosphate

• Effectors of protein activity:

• Interactions: Tkt-Tpi PubMed

• Localization:

Database entries

• BsubCyc: BSU17890

• Structure: [3K95], complex with thiamine diphosphate, from B. anthracis

• UniProt: P45694

• KEGG entry: [3]

• E.C. number: 2.2.1.1

Expression and regulation

• Operon: tkt PubMed

• Expression browser: tkt PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 7856 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 18478 PubMed

Biological materials

• Mutant: BS4530 (aphA3), available in Stülke lab

• Expression vector:
  • pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP820, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1406 (spc, based on pGP1331), available in the Stülke lab

References

Reviews

G Schenk, R G Duggleby, P F Nixon
Properties and functions of the thiamin diphosphate dependent enzyme transketolase.
Int J Biochem Cell Biol: 1998, 30(12);1297-318
[PubMed:9924800] [WorldCat.org] [DOI] (P p)