PyrC

• Description: dihydroorotase
  
  

Gene name	pyrC
Synonyms
Essential	no
Product	dihydroorotase
Function	pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrC
Metabolic function and regulation of this protein in SubtiPathways: pyrC
MW, pI	46 kDa, 5.427
Gene length, protein length	1284 bp, 428 aa
Immediate neighbours	pyrB, pyrAA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

• Locus tag: BSU15500

Database entries

• BsubCyc: BSU15500

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-dihydroorotate + H₂O = N-carbamoyl-L-aspartate (according to Swiss-Prot)

• Protein family: Type 2 subfamily (according to Swiss-Prot)

• Paralogous protein(s): PucH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU15500

• Structure: 3GRI (from Staphylococcus aureus subsp. aureus mw2, 60% identity, 72% similarity)

• UniProt: P25995

• KEGG entry: [3]

• E.C. number: 3.5.2.3

Expression and regulation

• Operon: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE PubMed

• Expression browser: pyrC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the absence of uridine nucleotides (PyrR) PubMed

• Regulatory mechanism:
  • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1551 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 5328 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yu-He Liang, Xiangyu Liu, Juan Wang, Lanfen Li, Xiao-Dong Su
Protein preparation, crystallization and preliminary X-ray crystallographic studies of dihydroorotase from Bacillus subtilis.
Protein Pept Lett: 2005, 12(7);717-9
[PubMed:16522191] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)