MurE

• Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelate synthetase
  
  

Gene name	murE
Synonyms
Essential	yes PubMed
Product	UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl- meso-2,6-diaminopimelate synthetase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murE
Metabolic function and regulation of this protein in SubtiPathways: murE
MW, pI	54 kDa, 5.558
Gene length, protein length	1482 bp, 494 aa
Immediate neighbours	spoVD, mraY
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU15180

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15180

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate (according to Swiss-Prot)

• Protein family: MurE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15180

• Structure: 2WTZ (from Mycobacterium tuberculosis, 35% identity, 50% similarity) PubMed

• UniProt: Q03523

• KEGG entry: [3]

• E.C. number: 6.3.2.13

Expression and regulation

• Operon: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed

• Expression browser: murE PubMed

• Sigma factor:

• Regulation:
  • expressed during vegatative growth PubMed
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 297 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1548 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
DNA sequence of the murE-murD region of Bacillus subtilis 168.
J Gen Microbiol: 1993, 139(2);361-70
[PubMed:8436954] [WorldCat.org] [DOI] (P p)

A O Henriques, H de Lencastre, P J Piggot
A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli.
Biochimie: 1992, 74(7-8);735-48
[PubMed:1391053] [WorldCat.org] [DOI] (P p)