SpoIISA

• Description: lethal when synthesized during vegetative growth in the absence of SpoIISB
  
  

Gene name	spoIISA
Synonyms	ykaC
Essential	no
Product	toxin
Function	programmed cell death
Gene expression levels in SubtiExpress: spoIISA
Interactions involving this protein in SubtInteract: SpoIISA
MW, pI	28 kDa, 4.683
Gene length, protein length	744 bp, 248 aa
Immediate neighbours	spoIISB, pit
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, toxins, antitoxins and immunity against toxins, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU12830

Database entries

• BsubCyc: BSU12830

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: three putative membrane-spanning segments and a cytoplasmic domain

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIISA-SpoIISB PubMed

• Localization:
  • cell membrane PubMed

Database entries

• BsubCyc: BSU12830

• Structure: 3O6Q (cytoplasmic fragment of SpoIISA (CSpoIISA) in complex with SpoIISB) PubMed

• UniProt: O34853

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: spoIISA-spoIISB PubMed

• Expression browser: spoIISA PubMed

• Sigma factor:

• Regulation:
• levels increase at the point of entry into stationary phase of cell cultures grown in sporulation-inducing medium, this is independent of Spo0A PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S123 ( spoIISA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jana Makroczyová, Stanislava Rešetárová, Patrik Florek, Imrich Barák
Topology of the Bacillus subtilis SpoIISA protein and its role in toxin-antitoxin function.
FEMS Microbiol Lett: 2014, 358(2);180-7
[PubMed:25039482] [WorldCat.org] [DOI] (I p)

Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767] [WorldCat.org] [DOI] (I p)

Stanislava Rešetárová, Patrik Florek, Katarína Muchová, Anthony J Wilkinson, Imrich Barák
Expression and localization of SpoIISA toxin during the life cycle of Bacillus subtilis.
Res Microbiol: 2010, 161(9);750-6
[PubMed:20863891] [WorldCat.org] [DOI] (I p)

Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016] [WorldCat.org] [DOI] (P p)

E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520] [WorldCat.org] [DOI] (P p)