Difference between revisions of "ConE"

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* '''Description:''' required for conjugation of ICE''Bs1'', part of the type IV secretion system for DNA transfer  <br/><br/>
  
  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
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|style="background:#ABCDEF;" align="center"| '''Product''' || conjugation protein
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|style="background:#ABCDEF;" align="center"| '''Product''' || part of the type IV secretion system for DNA transfer 
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || conjugative transfer of ICEBs1
 
|style="background:#ABCDEF;" align="center"|'''Function''' || conjugative transfer of ICEBs1

Revision as of 16:05, 28 May 2015

  • Description: required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer


Gene name conE
Synonyms yddE
Essential no
Product part of the type IV secretion system for DNA transfer
Function conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: conE
MW, pI 96 kDa, 5.511
Gene length, protein length 2493 bp, 831 aa
Immediate neighbours yddD, yddF
Sequences Protein DNA DNA_with_flanks
Genetic context
YddE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ConE expression.png















Categories containing this gene/protein

mobile genetic elements

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU04940

Phenotypes of a mutant

Defective for conjugal transfer of ICEBs1

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: required for conjugative transfer of ICEBs1, putative ATPase PubMed
  • Protein family: HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • Located at the cell poles, in close association with the membrane

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Melanie Barker Berkmen, Suffolk University, Boston, MA Homepage

Your additional remarks

References

Melanie B Berkmen, Catherine A Lee, Emma-Kate Loveday, Alan D Grossman
Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
J Bacteriol: 2010, 192(1);38-45
[PubMed:19734305] [WorldCat.org] [DOI] (I p)