Difference between revisions of "OpuAC"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 272 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 603 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:36, 17 April 2014

Gene name opuAC
Synonyms
Essential no
Product glycine betaine ABC transporter (binding protein)
Function compatible solute transport
Gene expression levels in SubtiExpress: opuAC
Interactions involving this protein in SubtInteract: OpuAC
Metabolic function and regulation of this protein in SubtiPathways:
opuAC
MW, pI 32 kDa, 8.015
Gene length, protein length 879 bp, 293 aa
Immediate neighbours opuAB, amhX
Sequences Protein DNA DNA_with_flanks
Genetic context
OpuAC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OpuAC expression.png















Categories containing this gene/protein

ABC transporters, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

RemA regulon

The gene

Basic information

  • Locus tag: BSU03000

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binding for subsequent uptake of glycine betaine PubMed
    • binding for subsequent uptake of dimethylglycine PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • KD for glycine betaine: 26 µM PubMed
    • KD for dimethylglycine: 172 µM PubMed
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2B4L (complex with glycine betaine), 3CHG (in complex with DMSA)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by osmotic stress PubMed
    • repressed by intracellular glycine betaine PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 272 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 603 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References