Difference between revisions of "MurAA"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36760&redirect=T BSU36760] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murAA.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murAA.html] | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36760&redirect=T BSU36760] | ||
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3SG1 3SG1] (from ''B. anthracis'', 79% identity, 94% similarity) | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3SG1 3SG1] (from ''B. anthracis'', 79% identity, 94% similarity) | ||
Revision as of 15:02, 2 April 2014
- Description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
| Gene name | murAA |
| Synonyms | murA |
| Essential | yes PubMed |
| Product | UDP-N-acetylglucosamine 1-carboxyvinyltransferase |
| Function | peptidoglycan precursor biosynthesis |
| Gene expression levels in SubtiExpress: murAA | |
| Metabolic function and regulation of this protein in SubtiPathways: murAA | |
| MW, pI | 46 kDa, 5.45 |
| Gene length, protein length | 1308 bp, 436 aa |
| Immediate neighbours | spoIID, ywmB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36760
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU36760
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)
- Protein family: MurA subfamily (according to Swiss-Prot)
- Paralogous protein(s): MurAB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU36760
- Structure: 3SG1 (from B. anthracis, 79% identity, 94% similarity)
- UniProt: P70965
- KEGG entry: [3]
- E.C. number: 2.5.1.7
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- pRSETA-murAA, Expression of His(6)-MurAA, avalable in Ulf Gerth's lab PubMed
- pGP2597: (IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
