Difference between revisions of "MetK"

From SubtiWiki
Jump to: navigation, search
Line 58: Line 58:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU30550&redirect=T BSU30550]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/metK.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/metK.html]
Line 92: Line 93:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU30550&redirect=T BSU30550]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1FUG 1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) {{PubMed|8723769}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1FUG 1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) {{PubMed|8723769}}

Revision as of 14:34, 2 April 2014

  • Description: S-adenosylmethionine synthetase

Gene name metK
Synonyms metE
Essential yes PubMed
Product S-adenosylmethionine synthetase
Function S-adenosylmethionine synthesis
Gene expression levels in SubtiExpress: metK
Metabolic function and regulation of this protein in SubtiPathways:
metK
MW, pI 43 kDa, 5.025
Gene length, protein length 1200 bp, 400 aa
Immediate neighbours asnB, pckA
Sequences Protein DNA DNA_with_flanks
Genetic context
MetK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MetK expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes, most abundant proteins

This gene is a member of the following regulons

S-box

The gene

Basic information

  • Locus tag: BSU30550

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
  • Protein family: AdoMet synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1FUG (from Escherichia coli, 63% identity, 75% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP432 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

Brooke A McDaniel, Frank J Grundy, Vineeta P Kurlekar, Jerneja Tomsic, Tina M Henkin
Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.
J Bacteriol: 2006, 188(10);3674-81
[PubMed:16672621] [WorldCat.org] [DOI] (P p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)