Difference between revisions of "Hag"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35360&redirect=T BSU35360]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/hag.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/hag.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35360&redirect=T BSU35360]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3A5X 3A5X] (from ''Salmonella typhimurium'', 42% identity)
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3A5X 3A5X] (from ''Salmonella typhimurium'', 42% identity)

Revision as of 14:55, 2 April 2014

  • Description: flagellin protein, about 20,000 subunits make up one flagellum

Gene name hag
Synonyms
Essential no
Product flagellin protein
Function motility and chemotaxis
Gene expression levels in SubtiExpress: hag
Interactions involving this protein in SubtInteract: Hag
MW, pI 32 kDa, 4.782
Gene length, protein length 912 bp, 304 aa
Immediate neighbours yvyC, csrA
Sequences Protein DNA DNA_with_flanks
Genetic context
Hag context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Hag expression.png















Categories containing this gene/protein

motility and chemotaxis, most abundant proteins

This gene is a member of the following regulons

CodY regulon, CsrA regulon, ScoC regulon, SigD regulon

The gene

Basic information

  • Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial flagellin family (according to Swiss-Prot)
  • Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 3A5X (from Salmonella typhimurium, 42% identity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Biological materials

  • Expression vector:
  • GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Jörg Stülke's lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Tony Romeo, Christopher A Vakulskas, Paul Babitzke
Post-transcriptional regulation on a global scale: form and function of Csr/Rsm systems.
Environ Microbiol: 2013, 15(2);313-24
[PubMed:22672726] [WorldCat.org] [DOI] (I p)


Original Publications