Difference between revisions of "YwbD"

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(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:''' ''amyE::p(ywbD-lacZ cat)'', constructed with [[pAC5]], available in [[ Jörg Stülke]]'s lab
+
* '''lacZ fusion:''' GP1614 ''amyE::p(ywbD-lacZ cat)'', constructed with pGP2150 based on [[pAC5]], available in [[ Jörg Stülke]]'s lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 11:31, 31 January 2014

  • Description: unknown

Gene name ywbD
Synonyms ipa-19d
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ywbD
MW, pI 44 kDa, 9.178
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours ywbE, ywbC
Sequences Protein DNA DNA_with_flanks
Genetic context
YwbD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwbD expression.png















Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

SinR regulon

The gene

Basic information

  • Locus tag: BSU38360

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing C(5)-methylcytosine (according to Swiss-Prot)
  • Protein family: RlmI family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: GP1614 amyE::p(ywbD-lacZ cat), constructed with pGP2150 based on pAC5, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695] [WorldCat.org] [DOI] (P p)