Difference between revisions of "ScpB"

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* '''Description:''' DNA segregation and condensation protein <br/><br/>
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* '''Description:''' part of the [[condensin]] complex, chromosomal origin condensation and segregation <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA segregation and condensation protein  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA segregation and condensation protein  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || maintenance of chromosome structure
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|style="background:#ABCDEF;" align="center"|'''Function''' || segregation of replication origins
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23210 scpB]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23210 scpB]
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* inviable during rapid growth {{PubMed|24440393}}
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* ''[[scpB]]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth {{PubMed|24440399,24440393}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
−
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
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* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
 
* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
** part of the [[condensin]] complex {{PubMed|24440399,24440393}}
 
** forms dimers {{PubMed|22385855}}
 
** forms dimers {{PubMed|22385855}}
 
** [[ScpB]]-[[Smc]] {{PubMed|22385855,12100548}}, this interaction takes place only in the presence of [[ScpA]]  {{PubMed|22385855}}
 
** [[ScpB]]-[[Smc]] {{PubMed|22385855,12100548}}, this interaction takes place only in the presence of [[ScpA]]  {{PubMed|22385855}}
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* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  
 
** nucleoid (Multiple) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
 
** nucleoid (Multiple) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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** the ([[Smc]])2-[[ScpA]]-[[ScpB]] complex forms two bipolar assemblies on the chromosome, one in each cell half {{PubMed|23475963}}
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** the [[condensin]] ([[Smc]])2-[[ScpA]]-[[ScpB]] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [[Spo0J]] {{PubMed|24440393,23475963}}
  
 
=== Database entries ===
 
=== Database entries ===
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<pubmed> 22933559 22934648 </pubmed>
 
<pubmed> 22933559 22934648 </pubmed>
 
== Original publications ==
 
== Original publications ==
−
<pubmed>12100548,12065423,12421306,22385855, 16479537, 19450516 12897137 7934830 11948165  23353789 24440393 23475963</pubmed>
+
<pubmed>12100548,12065423,12421306,22385855, 16479537, 19450516 12897137 7934830 11948165  23353789 24440399,24440393 23475963</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:28, 16 February 2014

  • Description: part of the condensin complex, chromosomal origin condensation and segregation

Gene name scpB
Synonyms ypuH
Essential no
Product DNA segregation and condensation protein
Function segregation of replication origins
Gene expression levels in SubtiExpress: scpB
Interactions involving this protein in SubtInteract: ScpB
MW, pI 21 kDa, 4.25
Gene length, protein length 591 bp, 197 aa
Immediate neighbours ypuI, scpA
Sequences Protein DNA DNA_with_flanks
Genetic context
YpuH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ScpB expression.png















Categories containing this gene/protein

DNA condensation/ segregation

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU23210

Phenotypes of a mutant

  • scpB mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: scpB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

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